Electrochemical enzyme immunoassay for phenytoin by flow-injection analysis incorporating a redox coupling agent

Autor:	Halsall Hb, William R. Heineman, H T Tang
Rok vydání:	1991
Předmět:	Flow injection analysis Analyte Chromatography medicine.diagnostic_test Chemistry Biochemistry (medical) Clinical Biochemistry Analytical chemistry Ascorbic acid Electrochemistry Redox Matrix (chemical analysis) Immunoassay medicine Selectivity
Zdroj:	Clinical Chemistry. 37:245-248
ISSN:	1530-8561 0009-9147
DOI:	10.1093/clinchem/37.2.245
Popis:	Using phenytoin as a model analyte, we demonstrate an electrochemical enzyme immunoassay based on flow-injection analysis and incorporating 2,6-dichloroindophenol (DCIP) as a redox coupling agent. DCIP reacts with NADH to form NAD+ and DCIPH2, the reduced form of the coupling agent. The production of DCIPH2 is monitored at +250 mV vs Ag/AgCl. This low applied potential improves selectivity in the biological matrix, differentiating against components that are oxidizable at the more-positive potentials required for direct electrochemical detection of NADH. The kinetics-based assay also eliminates other common interferences, mainly from ascorbic acid and glutathione. This system does not require precolumns or analytical columns for isolation of the NADH response. Good agreement with a routine clinical laboratory procedure for phenytoin is obtained for clinical samples (r = 0.95), illustrating the feasibility of such an approach.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::74e44a27ad757f43f6788326ec4a8e00 https://doi.org/10.1093/clinchem/37.2.245 Zobrazit plný text záznamu