Directed evolution of the dioxygenase complex for the synthesis of furanone flavor compounds

Autor:	Tomas Hudlicky, Sergey A. Selifonov, Henry Garcia, Lisa M. Newman
Rok vydání:	2004
Předmět:	Ozonolysis Stereochemistry Organic Chemistry Diol Xylene Toluene dioxygenase Ring (chemistry) Directed evolution Biochemistry chemistry.chemical_compound chemistry Dioxygenase Drug Discovery Selectivity
Zdroj:	Tetrahedron. 60:729-734
ISSN:	0040-4020
Popis:	Herein we report a new preparation of 4-hydroxy-2,5-dimethyl-2,3-dihydrofuran-3-one, the flavor compound strawberry furanone, based on a ‘green’ approach with a minimum number of steps. The first step is an enzymatic dioxygenation of p -xylene to form cyclohexadiene- cis -diol, followed by ring opening via ozonolysis, and ring closure to form the furanone. In efforts to improve the efficiency of the enzymatic step, a directed evolution approach was taken to increase the substrate specificity and selectivity of the toluene dioxygenase enzyme system.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::74dfb8285d02ab4957268f7826efb4eb https://doi.org/10.1016/j.tet.2003.10.105 Zobrazit plný text záznamu Full Text from ScienceDirect