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COMPARATIVE MORPHOLOGICAL CHANGES IN SMOOTH MYOCYTES AND MACROFAGES CD16 IN PULMONARY ARTERY AND AORTA IN FETUSES AND NEWBORNS EXPOSED TO CHRONIC INTRAUTERINE HYPOXIASorokina I., Markovsky V., Kaluzhyna O., Pliten O., Hol’yeva N. Background. Oxygen deficiency, both acute and chronic, causes a variety of pathological changes in a number of organs and systems of fetuses and newborns. In particular, there are morphological changes in the vessels. Information in the available literature regarding the morphological state of smooth myocytes (SM) and cells expressing CD16 in the pulmonary artery (PA) and aorta of fetuses and newborns under such conditions of pregnancy is various and insufficient. Subjects and methods. The results of complex pathomorphological research of SM and macrophages CD16 of fetuses and newborns PA and aorta in chronic intrauterine hypoxia with the use of histological (staining with hematoxylin and eosin), immunohistochemical (MCA to Anti-Human Smooth Muscle Actin and MCA to CD16), morphometric, statistical methods are presented in the article. The investigation was performed on WAG line rats, observing all ethical norms and rules of handling of laboratory animals. The location of SM and macrophages CD16 in the wall of the PA and aorta in normal pregnancy and under oxygen deficiency, the density of their location in the field of vision (SM – ×1000, CD16 – ×600) was calculated. A comparative analysis of the morphological characteristics of the studied cells between mentioned vessels was also carried out. Results. The macroscopic examination of the wall of PA and aorta in the group of fetuses and newborns from mothers with physiological pregnancy with a magnifier (×3, 8 diopters) revealed that it was elastic, had a smooth ivorish intima. Smooth muscle cells in PA and aorta during immunohistochemical identification are determined in intima and media, as well as in the adventitial vessels of vessels. With hypoxic effect their number decreased in comparison with control group, and the number of macrophages – increased in both described vessels. Conclusions. The density of smooth myocytes (SM) location in the thickness of the wall of PA and aorta in the fetuses and newborns during the physiological course of pregnancy differs significantly from each other with dominance in the first vessel. Under the influence of experimental chronic intrauterine hypoxia in PA and aorta of fetuses and newborns a significant (p |
