Action of α-cyclodextrin on phospholipid assemblies

Autor: Catherine Ringard-Lefebvre, Geneviève Le Bas, Valérie Bernat, Sylviane Lesieur
Rok vydání: 2007
Předmět:
Zdroj: Journal of Inclusion Phenomena and Macrocyclic Chemistry. 57:113-119
ISSN: 1573-1111
0923-0750
DOI: 10.1007/s10847-006-9209-8
Popis: Interactions of α-cyclodextrin (α-CD) with dimyristoylphosphatidylcholine (DMPC) and Egg phosphatidylcholine (Egg-PC) were studied (i) by analyzing surface pressure-area isotherms and surface tension of phospholipid monolayers formed at the interface between air and α-CD aqueous solutions and (ii) by X-ray diffraction performed on fully hydrated α-CD/phospholipid binary mixtures. The cyclodextrin molecules strongly interact with the two-dimension phospholipid assembly. Their addition into the aqueous sub-phase leads to the removal of part of the phospholipids from the air-water interface: the higher the α-CD concentration, the higher the phospholipid depletion. This should preferentially involve interactions between cyclodextrin and the phosphatidylcholine head group as α-CD is water-soluble and not surface-active. At the three-dimension level, the bilayer packing of the phospholipid lamellar phase appears not affected by the presence of cyclodextrin as shown by X-ray scattering at small angles whereas wide-angle diffraction patterns reveal the formation of a crystalline phase organized in a pseudo-hexagonal lattice usually characteristic of α-CD dimers. These results point out that α-CD should interact with bilayer-forming phospholipid molecules but likely according to a process that would preserve intact at least a part of the multilamellar assembly.
Databáze: OpenAIRE
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