| Autor: |
Kornelia Ziegler, Max Frimmer |
| Rok vydání: |
1984 |
| Předmět: |
|
| Zdroj: |
Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. 805:174-180 |
| ISSN: |
0167-4889 |
| DOI: |
10.1016/0167-4889(84)90165-4 |
| Popis: |
Cyclosporin A at concentrations of more than 10 nM protects isolated hepatocytes against the action of phalloidin. Cyclosporin A at 100 nM inhibits the uptake of demethyl[ 3 H]phalloin by 50%, and at 5 μM also that of [ 14 C]cholate. This inhibition is independent of the preincubation period and is not reversed by washing the cells. With a 30–60-fold excess of cyclosporin A, affinity labeling of plasma membrane proteins using 12 μM [ 3 H]isothiocyanatobenzamido cholate was reduced to 40–60% of the control. These findings indicate that transport inhibition by cyclosporin A in liver cells cannot be explained by simple competition on the level of the membrane protein(s) involved. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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