Molecular Cloning, Sequencing and Expression of a Cdna Coding for Human Antithrombin III
| Autor: | A. Cravador, Albert Herzog, Andrew W. Bollen, R. Loriau, A Van Elsen, Marc Hoylaerts, B. Colau, Patrick Jacobs |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
Expression vector
cDNA library Antithrombin Biology Molecular cloning Molecular biology biological factors Insert (molecular biology) law.invention carbohydrates (lipids) Plasmid law hemic and lymphatic diseases Complementary DNA medicine Recombinant DNA cardiovascular diseases circulatory and respiratory physiology medicine.drug |
| DOI: | 10.1016/b978-0-08-033215-4.50040-6 |
| Popis: | A cDNA library prepared from human liver was screened for antithrombin III, using as probe a 25-base-long synthetic oligodeoxyribonucleotide. A Plasmid, pULB 1744, was identified carrying an insert of 1476 bp coding for antithrombin III. DNA sequence analysis indicated that this DNA codes for most of the signal peptide and for the complete mature antithrombin III molecule. Part of the DNA insert of pULB1744 was recloned into a Plasmid expression vector. The recombinant Plasmid expresses antithrombin III. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|