P04 In Vitro Evaluation of Buffy Coat Derived Platelet Concentrates in SSP+ Platelet Storage Medium
| Autor: | P. Cookson, M. Beard, C. Turner, V. Hancock, Rebecca Cardigan, M. Wiltshire, S. Bashir, Margaret Garwood, C. Pergande, S. Thomas, K. Smith |
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| Rok vydání: | 2006 |
| Předmět: | |
| Zdroj: | Transfusion Medicine. 16:26-26 |
| ISSN: | 1365-3148 0958-7578 |
| DOI: | 10.1111/j.1365-3148.2006.00694_4.x |
| Popis: | Introduction As part of the process to improve the safety and quality of the platelet supply, the NBS is evaluating the use of platelet storage media (PSM). The objective of this study was to produce a platelet concentrate (PC) containing 70% SSP+ and 30% plasma, whilst maintaining platelet yield, and to evaluate their in vitro function. Study Design. Buffy coats were prepared using the standard NBS method and four (ABO matched) were pooled together with 250 mL of SSP+. Centrifugation was performed using optimised conditions for PSM and an Optipress used to express the PC through a Pall Autostop LD filter into a Pall ELX storage bag. Ten PCs were prepared, and platelet quality was assessed using in vitro assays for functionality and activation on days 1, 5, 7 and 9 of storage. Results Parameter Preparation day 1 Specification Median (range) n = 10. Volume (mL) 307 (299–317) 150–350 (Local) Residual WBC (×106 U-1) 0.1 (0–0.2) 240 (Red Book) The assay results were compared to platelets stored in 100% plasma from a previous study. Day SSP+ Plasma 1 5 7 9 1 5 7 9 Median, n = 10, †P |
| Databáze: | OpenAIRE |
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