Evaluation of Laboratory-Produced Biosurfactant by Rhodotorula Species and Its Antifungal Activity
| Autor: | Maral Gharaghani, Ali Zarei Mahmoudabadi, Marzieh Halvaeezadeh |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
biology
Serial dilution Chemistry Microorganism Biodegradation Rhodotorula Antimicrobial biology.organism_classification 030226 pharmacology & pharmacy 01 natural sciences 0104 chemical sciences 010404 medicinal & biomolecular chemistry 03 medical and health sciences 0302 clinical medicine Potency Agar diffusion test Food science General Pharmacology Toxicology and Pharmaceutics Bacteria |
| Zdroj: | Jundishapur Journal of Natural Pharmaceutical Products. 15 |
| ISSN: | 2228-7876 1735-7780 |
| DOI: | 10.5812/jjnpp.11846 |
| Popis: | BackgroundBiosurfactants are amphiphilic surface-active compounds that are produced by several microorganisms, including bacteria and fungi. Biodegradability, low toxicity, application diversity, and functionality under extreme conditions characterize them from chemical biosurfactants. It is found thatRhodotorulaspecies, read yeasts, have high potency for biosurfactant production. Recently, antimicrobial activities of biosurfactants have been subjected to new antibiotic therapy.ObjectivesThe aim of the present study was to evaluate the biosurfactant production by the different strains of Rhodotorula species in laboratory conditions. In addition, the antifungal activity of produced biosurfactant was assessed against several saprophytic fungi.MethodsIn the present study, 54 strains of Rhodotorula including R. glutinis (48 strains), R. minuta (two strains), R. mucilaginosa (two strains), and Rhodotorula species (two strains) were screened for biosurfactant production. The biosurfactant was produced in Sabouraud dextrose broth medium and confirmed by specific tests. The antifungal assay was carried out by a disk diffusion method using serial dilutions of biosurfactant.ResultsIn the present study, although all tested strains were capable of producing biosurfactantin vitro, the degree of biosurfactant production varied among the strains. 7.4% of the strains had the highest (+5) biosurfactant activity while 16.7%, 29.5%, 25.8%, and 20.4% had +4, +3, +2, and +1, respectively. In the present study, all tested fungi were inhibited at 40 µL of the biosurfactant.ConclusionsRhodotorula species are appropriate organisms for the production of biosurfactants and R. glutinis strains have the greatest ability to produce biosurfactant among other species. Furthermore, our results demonstrated that the produced biosurfactant by R. glutinis presents a valuable potential for biopharmaceutical applications. |
| Databáze: | OpenAIRE |
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