Solid state fermentation, storage and viability of Streptomyces similanensis 9X166 using agro-industrial substrates against Phytophthora palmivora-induced black rot disease in orchids
Autor: | Yaovapa Aramsirirujiwet, Chatsuda Sakdapetsiri, Norifumi Shirasaka, Vichien Kitpreechavanich, Yasuhisa Fukuta, Shinji Tokuyama |
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Rok vydání: | 2018 |
Předmět: |
0106 biological sciences
0301 basic medicine Ammonium sulfate Bran Phytophthora palmivora 030106 microbiology food and beverages Biology Raw material Shelf life biology.organism_classification 01 natural sciences Husk 010602 entomology 03 medical and health sciences Horticulture chemistry.chemical_compound chemistry Solid-state fermentation Insect Science Fermentation Agronomy and Crop Science |
Zdroj: | Biocontrol Science and Technology. 29:276-292 |
ISSN: | 1360-0478 0958-3157 |
Popis: | Streptomyces similanensis 9X166 is known to be an antagonist of the black rot pathogen of orchids, Phytophthora palmivora. In this study, we investigated the production of highly viable S. similanensis 9X166 cells by solid state fermentation using agro-industrial substrates, and the shelf life of a S. similanensis 9X166 dried solid. Rice bran was found to be the most appropriate raw material for production of both viable cells and β-1,3-glucanase. A medium containing 12 g of rice bran and coconut husks at a ratio of 10:2, supplemented with 10 mL of mineral salts produced the highest number of viable cells and greatest level of β-1,3-glucanase. Ammonium sulfate was the most suitable nitrogen source, and an initial moisture content of 65% and a temperature of 30°C were found to be optimal conditions for the production of viable cells and β-1,3-glucanase. Storing the dried fermented solid under non-vacuum conditions resulted in the highest cell viability. The specific rate of degradation on viability... |
Databáze: | OpenAIRE |
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