Polyfunctional CD4+ T cells specific for Trypanosoma cruzi can be restored after in vitro treatment with IL-7 and IL-27
| Autor: | Maria Ailen Natale, Todd Minning, Maria Gabriela Alvarez, Bruno Lococo, Maria Cecilia Albareda, Graciela Bertocchi, Rick Tarleton, Susana Laucella |
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| Rok vydání: | 2019 |
| Předmět: | |
| Zdroj: | The Journal of Immunology. 202:190.60-190.60 |
| ISSN: | 1550-6606 0022-1767 |
| Popis: | Chronically Trypanosoma cruzi-infected subjects bear several features associated with immune exhaustion, including an impairment in the polyfunctionality of T cells over time and alterations in the IL-7/IL-7R and IL-27/IL-27R axis. Since IL-7 and IL-27 have several functions on different immune cells, gene expression analysis was conducted in total PBMCs in response to rhIL-7 and rhIL-27 in vitro. TBX21, EOMES, GZMB and MIG-cytokine-induced gene expression was low in patients with severe Chagas disease compared with those without cardiac involvement. IL-7-induced TBX21 expression positively correlated with the magnitude of T. cruzi-specific T-cell responses. We also assessed whether treatment of PBMCs of chronically T. cruzi infected subjects with IL-7/IL-27 could improve the quality of T. cruzi-specific T-cell responses. Polyfunctionality of CD4+ T cells was improved after treatment with IL-7 and IL-27, by increasing the production of IL-2 on CD4+ T cells producing two or three functions in response to T. cruzi antigens in patients with mild cardiac disease. These findings support that alterations in IL-7/IL-27 pathways in chronic Chagas disease are not only at the signal-transduction level but also at the transcriptional regulation level. In vitro treatment of PBMCs of patients in early clinical stages of chronic Chagas disease can restore T-cell function. |
| Databáze: | OpenAIRE |
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