Multiple cytokines for the detection of Mycobacterium tuberculosis infection in children with tuberculosis
| Autor: | Ertan Mayatepek, Bernhard Fleischer, Florian M. Marx, Norman Nausch, H Henckel, Marc Jacobsen, Christian Lundtoft, G Schulz |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Pulmonary and Respiratory Medicine Tuberculosis biology business.industry Cytokine expression Interleukin Diagnostic accuracy Tumour necrosis factor alpha medicine.disease biology.organism_classification Mycobacterium tuberculosis 03 medical and health sciences 030104 developmental biology Infectious Diseases Tuberculosis diagnosis Active tb Immunology Medicine business |
| Zdroj: | The International Journal of Tuberculosis and Lung Disease. 21:270-277 |
| ISSN: | 1027-3719 |
| DOI: | 10.5588/ijtld.16.0351 |
| Popis: | Setting Interferon-gamma (IFN-γ) release assays (IGRAs) play an important role in the diagnosis of Mycobacterium tuberculosis infection. However, in children with tuberculosis (TB), some studies have shown increased frequencies of false-negative or indeterminate IGRA results. Objective To analyse the spectrum of different cytokines to improve the diagnostic accuracy of IGRAs in latent tuberculous infection (LTBI) and active TB. Design We performed multiplex cytokine expression analysis of QuantiFERON® Gold In-Tube supernatants in children with active TB (n = 21) and disease-free contacts with (n = 15) and without LTBI (n = 12), to determine the sensitivity and specificity of the modified tests. Results Of 21 initial cytokines analysed, IFN-γ and six other candidates (interleukin [IL] 2, inducible protein 10 [IP-10], IL-13, IL-1α, tumour necrosis factor alpha [TNF-α] and granulocyte-macrophage colony-stimulating factor [GM-CSF]) were significantly more elevated in children with TB and those with LTBI than in the non-infected controls. Sensitivity and specificity were similar for IFN-γ and IL-2, but lower for the remaining candidates. Notably, a subset of candidates, including IP-10, showed M. tuberculosis antigen-induced specific expression in non-infected children. None of the candidates showed differences in expression between children with TB and those with LTBI. Conclusions Our results did not suggest that alternative IGRA cytokines can distinguish between children with active TB and those with LTBI. IFN-γ and IL-2 showed comparable capacity in diagnosing M. tuberculosis infection in our study groups. |
| Databáze: | OpenAIRE |
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