The Effects of Ionizing Radiation on Avian Erythrocytes

Autor:	Howard S. Ducoff, Susan W. Lee
Rok vydání:	1994
Předmět:	Radiation business.industry Biophysics Biology medicine.disease Molecular biology Hemolysis Ionizing radiation Red blood cell medicine.anatomical_structure Total dose medicine Radiation damage Radiology Nuclear Medicine and imaging Irradiation Dose rate Nuclear medicine business
Zdroj:	Radiation Research. 137:104
ISSN:	0033-7587
DOI:	10.2307/3578797
Popis:	The effects of ionizing radiation were examined in terminally differentiated cells using nucleated chicken erythrocytes (RBCs) as the model. We used a hemolytic assay to score radiation damage to RBCs. Chicken RBCs received 0 to 100 Gy of radiation at dose rate of 10 Gy/min. Radiation-induced hemolysis occurred in a dose-dependent manner but not immediately after irradiation. Hemolysis became apparent at 24 h after treatment. A threshold for radiation dose response was observed. At doses below 30 Gy, hemolysis in irradiated samples was indistinguishable from that in nonirradiated controls. A total dose of 100 Gy was used for the split-dose experiments. The results showed that chicken RBCs were able to repair radiation damage and that the half-time for maximum recovery was approximately 30 min at 36{degrees}C. Recovery from {gamma} radiation was also affected by the interfraction temperature. 36 refs., 7 figs.
Databáze:	OpenAIRE
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