A Species-specific Monoclonal Antibody System for Detecting the Remains of Field Slugs, Deroceras reticulatum (Muller) (Mollusca: Pulmonata), in Carabid Beetles (Coleoptera: Carabidae)

Autor:	W. O. C. Symondson, J. E. Liddell
Rok vydání:	1996
Předmět:	education.field_of_study Deroceras reticulatum Slug fungi Population Millipede Biology biology.organism_classification Pulmonata Predation Insect Science Botany education Agronomy and Crop Science Mollusca Invertebrate
Zdroj:	Biocontrol Science and Technology. 6:91-100
ISSN:	1360-0478 0958-3157
DOI:	10.1080/09583159650039557
Popis:	The field slug, Deroceras reticulatum (Muller), is probably the most damaging and widespread species of slug, causing severe economic losses in a broad range of crops in temperate zones throughout the world. Investigations into the role of predation in the population dynamics of this species required a biochemical system which was capable of identifying the remains of this slug in the crop contents of predators, and distinguishing them from those of other molluscs. A monoclonal antibody was developed (IgM isotype). This was capable of separating, in an enzyme-linked immunosorbent assay, D. reticulatum from the related D. caruanae (Pollonera) and all molluscs and other invertebrates tested, with the unexpected exceptions of New Zealand flatworms, Artioposthia triangulata (Deny) and the millipede Polymicrodon polydesmoides (Leach). Characterization of the antibody and assay demonstrated that the system could clearly detect as little as 11.6 ng of D. reticulatum protein in 200 mu l of buffer. Slug remains co...
Databáze:	OpenAIRE
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