FTA® card tool for sampling and rapid diagnosis of bacterial diseases from rainbow trout (Oncorhynchus mykiss) tissue

Autor: Ifakat Tulay Cagatay
Rok vydání: 2021
Předmět:
Zdroj: Aquaculture International. 30:419-428
ISSN: 1573-143X
0967-6120
DOI: 10.1007/s10499-021-00810-6
Popis: The collection of farmed fish samples and subsequent diagnosis of microbial pathogens can be difficult and time-consuming procedure under field conditions. Correct diagnosis and identification of bacterial diseases agents (Yersinia ruckeri, Flavobacterium psychrophilum, Lactococcus garvieae, and Vagococcus salmoninarum) are crucial for the effective treatment. In this study, the feasibility of using FTA® card (Flinders Technology Associates filter papers) for sampling infected rainbow trout (Oncorhynchus mykiss) tissues and significantly accelerated the molecular diagnosis of enteric redmouth disease, rainbow trout fry syndrome, bacterial coldwater disease, lactococcosis associated with fatal hemorrhagic septicemia, and vagococcosis were evaluated. We also examined and compared a practical and reliable extraction obtained from immobilized DNA on FTA® card with one commonly used commercial kit to diagnose bacterial pathogens in fish tissues. Results proved that the active working time of extracting DNA from FTA® card is requiring only 30 min and the cost is less than USA $ 0.5 for each eluted sample. A total of hundred extracted DNA from tissues on FTA® card were tested and showed successful PCR amplification of the aroA, murG, pLG, and pSa1 genes. In conclusion, our finding indicates FTA® card as an appropriate tool for the simple collection, easy transfer, and stored at room temperature without contamination and retrieval of high-quality DNA for rapid molecular diagnosis of bacterial fish pathogens without isolation and culture.
Databáze: OpenAIRE
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