The β-lactoglobulin content of bovine milk: Development and application of a biosensor immunoassay
| Autor: | David C Woollard, Theo Meerkerk, Selena Hart, Brendon D. Gill, Harvey E. Indyk |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
food.ingredient
01 natural sciences Applied Microbiology and Biotechnology fluids and secretions food Lactation Skimmed milk medicine Surface plasmon resonance Beta-lactoglobulin Detection limit Chromatography biology medicine.diagnostic_test Chemistry 010401 analytical chemistry 0402 animal and dairy science food and beverages 04 agricultural and veterinary sciences 040201 dairy & animal science 0104 chemical sciences medicine.anatomical_structure Immunoassay biology.protein Colostrum Biosensor Food Science |
| Zdroj: | International Dairy Journal. 73:68-73 |
| ISSN: | 0958-6946 |
| DOI: | 10.1016/j.idairyj.2017.05.010 |
| Popis: | An optical biosensor immunoassay exploiting surface plasmon resonance is described for the quantification of β-lactoglobulin in milk. Samples were diluted with buffer, and the protein estimated from binding with a polyclonal antibody immobilised on the sensor surface. Analytical method performance characteristics including range, detection limit, precision and accuracy were determined and reported. The temporal variability in the β-lactoglobulin content of milk from pasture-fed cows during early lactation and across a production season was investigated. The content of β-lactoglobulin decreased from >10 mg mL −1 in early colostrum to −1 in mature milk, and the β-lactoglobulin content of skim milk powder trended from 25 to 60 mg g −1 across a season. In view of its allergenicity, these data will improve understanding of the expression of innate β-lactoglobulin in the milk of pasture-grazed dairy herds, thereby providing information that is applicable to the formulation of bovine milk-based products. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect