Fe3O4/PVIM-Ni2+ Magnetic Composite Microspheres for Highly Specific Separation of Histidine-Rich Proteins
| Autor: | Yuting Zhang, Wanfu Ma, Changchun Wang, Dian Li, Meng Yu, Jia Guo |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Materials science
Histidine-rich proteins equipment and supplies Microsphere chemistry.chemical_compound Electrophoresis Affinity chromatography Chemical engineering chemistry Polymerization Organic chemistry Imidazole General Materials Science Selectivity human activities Polyacrylamide gel electrophoresis |
| Zdroj: | ACS Applied Materials & Interfaces. 6:8836-8844 |
| ISSN: | 1944-8252 1944-8244 |
| Popis: | Integration of the advantages of immobilized metal-ion affinity chromatography (IMAC) and magnetic microspheres is considered as an ideal pathway for quick and convenient separation of his-tagged proteins, but rare reports concern the natural histidine-rich proteins. In this article, a novel route was presented to fabricate magnetic microspheres composed of a high-magnetic-response magnetic supraparticle (Fe3O4) core and a Ni2+-immobilized cross-linked polyvinyl imidazole (PVIM) shell via reflux-precipitation polymerization. The unique as-prepared Fe3O4/PVIM-Ni2+ microspheres possessed uniform flower-like structure, high magnetic responsiveness, abundant binding sites, and very easy synthesis process. Taking advantage of the pure PVIM-Ni2+ interface and high Ni2+ loading amount, the microspheres exhibited remarkable selectivity, excellent sensitivity, large enrichment capacity, and high recyclability in immobilization and separation of his-tagged recombinant proteins. More interestingly, it was found that... |
| Databáze: | OpenAIRE |
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