Vasculogenesis and Diabetic Erectile Dysfunction: How Relevant Is Glycemic Control?
| Autor: | Pedro Vendeira, Carla Costa, Liliana Leite, Pedro Gomes, Luísa Guardão, Ilda Rodrigues, A. Castela, Ricardo Silvestre, Rui Chilro, Ronald Virag |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty medicine.medical_treatment CD34 030209 endocrinology & metabolism Biochemistry 03 medical and health sciences 0302 clinical medicine Vasculogenesis Diabetes mellitus Internal medicine medicine Endothelial dysfunction Progenitor cell Molecular Biology Glycemic 2. Zero hunger business.industry Insulin Cell Biology medicine.disease 3. Good health 030104 developmental biology Endocrinology Erectile dysfunction cardiovascular system business |
| Zdroj: | Journal of Cellular Biochemistry. 118:82-91 |
| ISSN: | 0730-2312 |
| DOI: | 10.1002/jcb.25613 |
| Popis: | Erectile dysfunction (ED) is a complication of diabetes, condition responsible for causing endothelial dysfunction (EDys) and hampering repair mechanisms. However, scarce information is available linking vasculogenesis mediated by Endothelial Progenitor Cells (EPCs) and diabetes-associated ED. Furthermore, it remains to be elucidated if glycemic control plays a role on EPCs functions, EPCs modulators, and penile vascular health. We evaluated the effects of diabetes and insulin therapy on bone marrow (BM) and circulating EPCs, testosterone, and systemic/penile Stromal Derived Factor-1 alpha (SDF-1α) expression. Male Wistar rats were divided into groups: age-matched controls, 8-weeks streptozotocin-induced type 1 diabetics, and insulin-treated 8-weeks diabetics. EPCs were identified by flow cytometry for CD34/CD133/VEGFR2/CXCR4 antigens. Systemic SDF-1α and testosterone levels were evaluated by ELISA. Penile SDF-1α protein expression was assessed, in experimental and human diabetic cavernosal samples, by immunohistochemical techniques. Diabetic animals presented a reduction of BM-derived EPCs and an increase in putative circulating endothelial cells (CECs) sloughed from vessels wall. These alterations were rescued by insulin therapy. In addition, glycemic control promoted an increase in systemic testosterone and SDF-1α levels, which were significantly decreased in animals with diabetes. SDF-1α protein expression was reduced in experimental and human cavernosal diabetic samples, an effect prevented by insulin in treated animals. Insulin administration rescued the effects of diabetes on BM function, CECs levels, testosterone, and plasmatic/penile SDF-1α protein expression. This emphasizes the importance of glycemic control in the prevention of diabetes-induced systemic and penile EDys, by the amelioration of endothelial damage, and increase in protective pathways. J. Cell. Biochem. 118: 82-91, 2017. © 2016 Wiley Periodicals, Inc. |
| Databáze: | OpenAIRE |
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