Isolation of Dental Pulp Stem Cells and their In Vitro Differentiation into Odontoblast-like Cells

Autor: Nermeen El-Motaz Bellah Ahmed, Siza Yacoub Zakhary, Magda Ismail Ramzy, Eman Hassan Anwar Aboul-Ezz, Tarek Hamed El Badry
Rok vydání: 2011
Předmět:
Zdroj: Macedonian Journal of Medical Sciences. 4:253-260
ISSN: 1857-5773
1857-5749
DOI: 10.3889/mjms.1857-5773.2011.0142
Popis: Background: Recently, tooth tissue engineering has attracted more and more attention. Stem cell based tissue engineering is thought to be a promising way to replace the missing tooth. Mesenchymal stem cells (MSCs) are multipotent stem cells which can differentiate into a variety of cell types. Material and Methods: We isolated stem cells from dental pulp and measured their self-renewal capacities. Adipogenic, chondrogenic as well as odontogenic differentiation potentials were investigated, using bone morphogenic protein 2 (BMP2) for the odontogenic differentiation. out. Results: The cumulative number of the isolated cells was high. Polycomb ring finger oncogene (Bmi1) and Signal transducer and activator of transcription 3 (Stat3) were continuously expressed suggesting longer proliferative lifespan and self-renewal capacity of the isolated cells. Peroxisome proliferator-activated receptor was expressed showing adipogenic conversion that was also confirmed by positive staining of cells with Oil red O stain and chondrogenic differentiation was confirmed by positive staining of cells with Alcian blue stain. BMP2 stimulated the expression of dentin sialophosphoprotein (DSPP) and enamelysin, indicating successful odontogenic differentiation that was also confirmed by the positive staining of the cells with Alizarin red stain. Conlusion: Thus, adult pulp contains stem cells, which are useful for cell therapy with BMP2 for dentin regeneration.
Databáze: OpenAIRE
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