Peptide synthesis on a phenolic resin support
| Autor: | D. Hudson, late G.W. Kenner |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
chemistry.chemical_classification
Tetrapeptide Chemistry technology industry and agriculture Peptide General Medicine Transesterification Condensation reaction Biochemistry Merrifield resin chemistry.chemical_compound Hydrolysis stomatognathic system Structural Biology Trifluoroacetic acid Peptide synthesis Organic chemistry Molecular Biology |
| Zdroj: | International Journal of Biological Macromolecules. 2:63-67 |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/0141-8130(80)90029-x |
| Popis: | Protected peptides assembled on a phenolic resin support were cleared by peroxide-catalysed hydrolysis. In genenal peptide phenyl ester resins were more labile to nucleophiles than were corresponding Merrifield resin derivatives; transesterification with dimethylaminoethanol providing on alternative cleavage method for peroxide-sensitive peptides. Losses of radiolabelled peptide from both Merrifield and phenolic resins were determined during acid deprotection, base wash and coupling steps in the synthesis of a tetrapeptide. Using 40% (v/v) trifluoroacetic acid in dichloromethane for Boc-deprotection the phenolic resin gave improved results compared to the Merrifield resin. The merits of the procedure for the preparation of protected peptide acids suitable for subsequent condensation reactions were exemplified by the synthesis of an octapeptide sequence of a modified lysozyme. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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