Effects of dietary protein levels on activities of protease and expression of ingestion and protein digestion‐related genes in Nile tilapia juveniles
Autor: | Walisson de Souza e Silva, Paula Adriane Perez Ribeiro, José Fernando López-Olmeda, N. C. S. Costa, Welliene Moreira dos Santos, Leandro Santos Costa, Priscila Vieira Rosa, Ronald Kennedy Luz, Pedro G. Gamarano, Fabio A. C. Santos |
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Rok vydání: | 2020 |
Předmět: |
0303 health sciences
Proteases Protease biology Protein digestion media_common.quotation_subject medicine.medical_treatment Proteolytic enzymes Appetite 04 agricultural and veterinary sciences Aquatic Science biology.organism_classification 03 medical and health sciences Nile tilapia Peptide YY 040102 fisheries medicine 0401 agriculture forestry and fisheries Food science Digestion 030304 developmental biology media_common |
Zdroj: | Aquaculture Research. 51:2973-2984 |
ISSN: | 1365-2109 1355-557X |
Popis: | While some studies have investigated the effects of dietary protein on the activities of protease in Nile tilapia, little information exists about these enzymes and hormones regulating appetite at the molecular level. Therefore, we investigated the performance, protease activities and expression of genes related to protein intake and digestion in juvenile tilapia fed with diets containing different levels of crude protein (CP): 25.14, 31.12, 36.60 and 42.05%. The fish were fed to apparent satiation three times a day for 65 days. The animal performance and the retention rates of protein and energy linearly decreased with an increase in dietary CP content. A positive quadratic effect of CP on pepsinogen was observed, although the acid protease activity was not affected. Trypsinogen and trypsin activity in the intestine presented similar patterns, showing a positive quadratic response to dietary protein levels. A linear increase in intestinal chymotrypsinogen expression was observed, but the activity of chymotrypsin showed a positive quadratic response. In addition, the expression of cholecystokinin (cck) and peptide yy (pyy) increased linearly with increasing CP. Dietary protein modulates the activity of alkaline proteases as well as the expression of these protease genes and hormones involved in appetite regulation. These changes can help expand our understanding of feed intake and digestion control, thereby improving feed formulations for this species. |
Databáze: | OpenAIRE |
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