Characterization of systemic, mucosal and lymphoid granulocyte subsets in rhesus macaques

Autor: Cordelia Manickam, Rhianna Jones, Daniel Ram, Kyle Kroll, Brady Hueber, Valerie Varner, R. Keith Reeves
Rok vydání: 2020
Předmět:
Zdroj: The Journal of Immunology. 204:92.2-92.2
ISSN: 1550-6606
0022-1767
Popis: Granulocytes mediate immune protection by phagocytosis, NETs, degranulation, antibody mediated effector functions and recruitment of innate and adaptive cells against pathogens. However, limited research describing granulocytes in HIV infection and in mucosal tissues exists. In particular, the role of granulocytes in the rhesus macaque (RM) model of HIV infection and vaccine/treatment studies have been understudied. Our goal was to characterize granulocyte subsets in systemic, mucosal and lymphoid tissues of naïve and infected RM. Mononuclear cells from jejunum, colon, cervix, vagina, lymph nodes, spleen, liver, and whole blood of naïve, SIV+, and SHIV+ RM were characterized using flow cytometry and Imagestream. Phenotype of granulocyte subsets were identified as- CD45+CD66+CD49d+ eosinophils, CD45+CD66+CD14+ neutrophils, and CD45+CD123+FcRE+ basophils. Polynuclear and mononuclear visualization with DAPI staining and surface marker images by Imagestream analysis further confirmed granulocytic phenotypes. Flow cytometry data showed that differential expression of FcRgs (CD64, CD32 and CD16) can be helpful in distinguishing granulocyte subsets while FcRE was only expressed by basophils. Interestingly, frequencies of granulocyte populations were generally higher in mucosal compared to lymphoid tissues in both naïve and infected animals. Eosinophils were particularly enriched within jejunal tissue, with a median of 45% of CD45+ cells. High frequencies of granulocytes in mucosal tissues indicate their importance in gut and reproductive tract immunity. Overall, further investigation is warranted to understand the significance of granulocytes in tissue homeostasis and infection.
Databáze: OpenAIRE
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