A NEW METHOD FOR PURIFYING COXIELLA BURNETII BY DEXTRAN SULFATE PRECIPITATION
| Autor: | William C. Patrick, Jack L. Davis, Iii. |
|---|---|
| Rok vydání: | 1964 |
| Předmět: |
animal structures
Chromatography biology Precipitation (chemistry) Sodium chemistry.chemical_element Calcium bacterial infections and mycoses Chick embryos Coxiella burnetii biology.organism_classification Microbiology chemistry.chemical_compound Dextran sulfate Dextran chemistry embryonic structures bacteria Centrifugation |
| DOI: | 10.21236/ad0452977 |
| Popis: | This paper describes a simple method for purifying Coxiella burnetii cultivated in chick embryos. The procedure is based upon the preliminary clarification of infected whole-egg suspensions by precipitating the low-density lipoproteins and other extraneous solids using dextran sulfate, calcium chloride, and sodium chloride solutions, then concentrating the rickettsiae by centrifugation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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