Lightmicroscopical histochemistry of hydrolases in the root tip of lima beans,Phaseolus lunatus L., in relation to cell differentiation and wound regeneration

Autor:	M. Ruddat, J. R. Esterly, Claire V. Thomas
Rok vydání:	1977
Předmět:	biology Acid phosphatase Dehydrogenase Cell Biology Plant Science General Medicine biology.organism_classification Aminopeptidase Molecular biology food.food Rhizodermis food Biochemistry biology.protein Lima beans Phaseolus Leucine Root cap
Zdroj:	Protoplasma. 91:229-242
ISSN:	1615-6102 0033-183X
DOI:	10.1007/bf01281948
Popis:	Acid phosphatase, Β-glucosidase, Β-galactosidase, glucosaminidase, nonspecific esterase and leucine aminopeptidase were determined histochemically in Lima bean root tips. Highest enzyme activities were observed in terminally differentiating cells, such as xylem and root cap cells and also in the rhizodermis. Meristematic and parenchymatic cells contained the lowest hydrolase activity. The histochemical enzyme pattern of developing lateral roots resembled in all details that of the main root. tip. Regenerating root tissue contained increased levels of acid phosphatase, glucose-6-phosphate dehydrogenase, Β-glucosidase, naphthol-Β-galactosidase and nonspecific esterase. Changes in the activity of indoxyl-Β-galactosidase, Β-glucosaminidase, and leucine aminopeptidase were not observed during wound regeneration. Cell viability was monitored histochemically with succinic dehydrogenase, glucose-6-phosphate dehydrogenase, and cytochrome C oxidase.
Databáze:	OpenAIRE
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