Improvement of the bio-functional properties of TiO2 nanotubes

Autor: Lionel Marcon, Mariusz Andrzejczuk, Marcin Holdynski, Marcin Pisarek, Maria Janik-Czachor, Agata Roguska, Anna Belcarz
Rok vydání: 2016
Předmět:
Zdroj: Applied Surface Science. 388:775-785
ISSN: 0169-4332
DOI: 10.1016/j.apsusc.2016.03.128
Popis: Titanium oxide nanotubes with diameters from ca. 40–120 nm fabricated by the anodic oxidation of Ti at a constant voltage (10–28 V) were investigated to identify factors improving their bio-functional properties. Prepared substrates were subsequently annealed at 450 °C and 650 °C to obtain nanotubes having a crystalline structure, and were then examined by SEM, XRD, XPS, BET and contact angle measurement techniques. The thermally stabilized surfaces were subjected to bidirectional functionalization: by deposition of a thin layer of Ca-P and by loading with silver nanoparticles. Three factors were found to promote the proliferation of osteoblast (U2OS) cells: a larger nanotube diameter, a higher annealing temperature, and the presence of a thin Ca-P layer. Differentiation of these cells (by ALP test) was stimulated by a higher (650 °C) nanotube annealing temperature, but not by a thin Ca-P layer. The TiO2 nanotubes-modified samples exhibited noticeable antibacterial properties. Moreover, the additional deposition of Ag nanoparticles almost completely inhibited the survivability of S. epidermidis cells beyond 3 h of contact. In conclusion, TiO2 nanotubes-modified surfaces exhibit distinct bone forming ability and significant antibacterial properties, and can be easily functionalized by a thin Ca-P layer or nano-Ag deposition for further improvement of the above functionalities.
Databáze: OpenAIRE
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