Culture of Spontaneously Contracting Myocardial Cells from Adult Rats

Autor:	Stuart L. Jacobson
Rok vydání:	1977
Předmět:	chemistry.chemical_classification Physiology Cell Biology General Medicine Anatomy Trypsin Isolation (microbiology) Enzyme chemistry Biochemistry Collagenase medicine Molecular Biology Electric stimulation medicine.drug
Zdroj:	Cell Structure and Function. 2:1-9
ISSN:	1347-3700 0386-7196
DOI:	10.1247/csf.2.1
Popis:	A method is described for isolating single myocardial cells from adult rats and for establishing them in culture. Isolation is achieved by simul-taneous enzymatic and mechanical treatment of excised cardiac tissue. Exposure to the enzymes, a mixture of trypsin and collagenase, is carried out in a specially designed apparatus in which enzyme-loosened cells are liberated from the tissue. Cells are spontaneously contractile upon isolation, establish in culture, and continue to contract for up to 60 days. Features of the isolation procedure are discussed and are compared with those of conventional procedures. Preliminary results are reported showing that isolated cells are sensitive to electric stimulation and to application of L-epinephrine.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::73d4f0fc6fcd5446cae879245f9ba33f https://doi.org/10.1247/csf.2.1 Zobrazit plný text záznamu