Cost-effective assays for use in monitoring carbamazepine, phenobarbital, and phenytoin in serum

Autor:	F. M. L. Moore, D. Simpson
Rok vydání:	1989
Předmět:	Phenytoin Chromatography medicine.diagnostic_test Chemistry Biochemistry (medical) Clinical Biochemistry Carbamazepine Negative bias Therapeutic drug monitoring Immunoenzyme techniques medicine In patient Phenobarbital Repeat analysis medicine.drug
Zdroj:	Clinical Chemistry. 35:1782-1784
ISSN:	1530-8561 0009-9147
DOI:	10.1093/clinchem/35.8.1782
Popis:	Using Syva "EMIT" reagents and the Cobas Bio centrifugal analyzer, we have developed cost-effective assays for therapeutic drug monitoring of carbamazepine, phenobarbital, and phenytoin. With these optimized methods one can assay up to 2470 samples with a single 100-test EMIT kit with good precision and accuracy. Between-batch CVs, based on results of repeat analysis of routine patients' samples, were 4.6%, 7.0%, and 5.3%, respectively. Results correlated well with those by methods previously used [gas chromatography and fluorescence polarization (Abbott TDx)], but there was a negative bias (-10.9%) of our method for phenobarbital results in patients' samples, as compared with those obtained with the TDx.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::73d1d1b83f16acfeff0d4e630803678d https://doi.org/10.1093/clinchem/35.8.1782 Zobrazit plný text záznamu