Ocular Toxicity of Intravitreal Tacrolimus
| Autor: | Elke Passos, Gholam A. Peyman, Mahmoud Genaidy |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
medicine.medical_specialty
Retina Lagomorpha genetic structures medicine.diagnostic_test biology business.industry Balanced salt solution Retinal biology.organism_classification eye diseases Tacrolimus Surgery Ophthalmoscopy chemistry.chemical_compound medicine.anatomical_structure chemistry Ophthalmology Toxicity medicine sense organs business Electroretinography |
| Zdroj: | Ophthalmic Surgery, Lasers and Imaging Retina. 33:140-144 |
| ISSN: | 2325-8179 2325-8160 |
| Popis: | * BACKGROUND AND OBJECTIVE: To investigate the ocular toxicity of intravitreally administered tacrolimus, a drug with potent immunosuppressive activity. * METHODS: To evaluate toxicity, tacrolimus was injected into the mídvitreous cavity of 20 eyes of New Zealand pigmented rabbits at concentrations of 10, 50, 100, 250, 500, and 1000 µg. Control eyes received balanced salt solution. Eyes receiving 1000 µg were given injections of 0.2 mL solution; all others, including controls, received 0.1 mL. Rabbits were examined before the injections by slit-lamp biomicroscopy, indirect ophthalmoscopy, and an electroretinography test (ERG) was performed. The animals were followed up to 14 days postinjection by clinical examination and ERG. The animals were killed and the eyes were enucleated and processed for light microscopy. * RESULTS: No evidence of a retinal toxic reaction was seen in the eyes receiving 10 or 50 µg of tacrolimus. One out of 4 eyes that received 1 OO pg of the drug developed a vitreous reaction. All eyes treated with 250 pg or more developed vitreous reaction. One eye injected with 1000 µg of the drug developed occlusion of the temporal retinal vessels. Electroretinography showed decreasing b-wave amplitude with both dark- and lightadapted stimulus in the 500 and 1 000 µg groups, and it was normal in the other groups. Histopathologic sections showed mild disorganization of the retina only at the 500 and 1000 pg dosage. * CONCLUSIONS: Doses of 10 and 50 ug of tacrolimus are nontoxic to rabbit eyes. Only transient vitreous opacities were observed in the groups that received 100 and 250 pg. Intravitreal doses of 500 and 1000 pg of tacrolimus proved to be toxic to the retina. [Ophthalmic Surg Lasers 2002;33: 140-144] |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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