| Autor: |
Andreas Esch, Harald Kosegarten, Konrad Mengel, H. E. Goldbach, Franz Grolig, K-H. Glüsenkamp |
| Rok vydání: |
1997 |
| Předmět: |
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| Zdroj: |
Boron in Soils and Plants ISBN: 9789401063449 |
| DOI: |
10.1007/978-94-011-5564-9_44 |
| Popis: |
A fluorescein boronic acid conjugate has been used as a pH-sensitive dye in the root apoplast and because of its likely links to boric acid-binding sites. The marker (Glusenkamp et al.; German Patent Application) is non-toxic to cell growth up to concentrations of 100 μM and stains almost exclusively the apoplastic space of sunflower and maize roots. The meristematic zone behind the calyptra is hardly stained, which is in line with the assumption of a lack of demand for boron (B) during cell divison. By contrast, the expansion zone shows a more intense staining in a part of the longitudinal walls which is indicative of an intensive secretion of B-binding cell wall material into this part. A ‘banding’ of the stain in sunflower roots hints to a pulsating extension growth or at least intermittent secretion of B binding moieties. Root hairs seem to be more intensively stained than other cells of this region, especially at their tips. Experiments are under way to further characterize the bonds and to apply other boronic acid-coupled dyes for the histological identification of boric acid-binding ligands. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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