A conductive crosslinked graphene/cytochrome c networks for the electrochemical and biosensing study
| Autor: | A.K.M. Kafi, Maxwell J. Crossley, Mohammad Choucair, Mashitah M. Yusoff |
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| Rok vydání: | 2017 |
| Předmět: |
Chemistry
Inorganic chemistry Analytical chemistry 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology Condensed Matter Physics Electrochemistry 01 natural sciences Reference electrode Amperometry 0104 chemical sciences Quinhydrone electrode Electrode Palladium-hydrogen electrode Reversible hydrogen electrode General Materials Science Electrical and Electronic Engineering 0210 nano-technology Biosensor |
| Zdroj: | Journal of Solid State Electrochemistry. 21:2761-2767 |
| ISSN: | 1433-0768 1432-8488 |
| DOI: | 10.1007/s10008-017-3598-z |
| Popis: | The direct electrochemistry of catalytically active cytochrome C (Cyt c) adsorbed together with a 3-dimensional network of chemically synthesized graphene on glassy carbon electrode has been readily obtained in aqueous phosphate buffer. Direct electrical communication between the redox center of Cyt c and the modified graphene-based electrode was established. The modified electrode was employed as a high-performance hydrogen peroxide (H2O2) biosensor. The Cyt c present in modified electrode exhibited a pair of quasi-reversible redox peaks with a midpoint potential of −0.380 and −0.2 V, cathodic and anodic, respectively. Investigations into the electrocatalytic activity of the modified electrode upon hydrogen peroxide exposure revealed a rapid amperometric response (5 s). Under optimized conditions, the linear range of response to H2O2 concentration ranged from 5 × 10−7 to 2 × 10−4 M with a detection limit of 2 × 10−7 M at a signal-to-noise ratio of 3. The stability, reproducibility, and selectivity of the proposed biosensor are discussed in relation to the morphology and composition of the modified electrode. |
| Databáze: | OpenAIRE |
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