A blind comparison of simple bench-top bioassays and human tumour cell cytotoxicities as antitumor prescreens
| Autor: | M. Suffness, J. E. Anderson, C. M. Goetz, Jerry L. McLaughlin |
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| Rok vydání: | 1991 |
| Předmět: |
biology
Chemistry fungi Cell Cancer Brine shrimp Plant Science General Medicine biology.organism_classification medicine.disease Biochemistry Molecular biology In vitro Analytical Chemistry medicine.anatomical_structure Complementary and alternative medicine In vivo Drug Discovery medicine Molecular Medicine Bioassay Artemia salina Cytotoxicity Food Science |
| Zdroj: | Phytochemical Analysis. 2:107-111 |
| ISSN: | 1099-1565 0958-0344 |
| DOI: | 10.1002/pca.2800020303 |
| Popis: | Simple bench-top bioassays involving brine shrimp lethality, Lemna frond proliferation, and the inhibition of crown gall tumours on potato discs, as well as the human tumour cell lines A-549 lung carcinoma, MCF-7 breast carcinoma and HT-29 colon adenocarcinoma, were compared for their accuracy to detect known in vivo (P-388) active antitumour agents supplied by the National Cancer Institute. The potato disc assay was the best and showed excellent correlation to in vivo activity (p=0.008). The brine shrimp assay (p = 0.033) proved to be superior or equally as accurate as the in vitro human solid tumour cell lines (p = 0.033-0.334). The Lemna assay (p = 0.708) showed the poorest correlation. The brine shrimp and potato disc assays are suggested as convenient in-house prescreens to existing cytotoxicity or antitumour assays. |
| Databáze: | OpenAIRE |
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