Enzymatic synthesis of palm-based ascorbyl esters

Autor:	Noorullhamezon Md. Noor, Hamidah Burham, Hamidah Sidek, Suzaini Badruddin, Raizatul Ainaa Gafoor Abdul Rasheed
Rok vydání:	2009
Předmět:	Chromatography Antioxidant biology DPPH Process Chemistry and Technology medicine.medical_treatment Bioengineering Alcohol Transesterification Ascorbic acid Biochemistry Catalysis chemistry.chemical_compound chemistry biology.protein medicine Organic chemistry Lipase Palm
Zdroj:	Journal of Molecular Catalysis B: Enzymatic. 58:153-157
ISSN:	1381-1177
Popis:	The synthesis of palm-based ascorbyl esters through transesterification of ascorbic acid and palm oil in tert-amyl alcohol catalyzed by immobilized lipase is described. Highest conversion (70–75%) was determined after 16 h reaction at 40 °C using lipase (Novozyme 435 from Candida antartica ) with an ascorbic acid to palm oil mole ratio of 1:8. The purified product was further characterized by 13 C NMR and GC–MS and the mixture of ascorbyl monoesters obtained were identified as ascorbyl monooleate (61%), ascorbyl monopalmitate (30%) and ascorbyl monostearate (9%). The antioxidant activity of palm-based ascorbyl esters was evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) test. The results showed that pure palm-based ascorbyl esters have an antioxidant activity with an IC 50 value of 0.1 mg/mL.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::737b7b7f83c9aef247c3601855f580b5 https://doi.org/10.1016/j.molcatb.2008.12.012 Zobrazit plný text záznamu Full Text from ScienceDirect