Bestimmung von stickstoffhaltigen Pesticiden durch Hochleistungsflüssigkeits-Chromatographie mit Diodenarray-Detektion
| Autor: | R. Reupert, E. Plöger |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Detection limit
Chromatography Absorption spectroscopy Chemistry Clinical Biochemistry Phosphate buffered saline Extraction (chemistry) General Medicine High-performance liquid chromatography Analytical Chemistry Column chromatography Gradient elution General Materials Science Absorption (chemistry) |
| Zdroj: | Fresenius' Zeitschrift für analytische Chemie. 331:503-509 |
| ISSN: | 0016-1152 |
| DOI: | 10.1007/bf00467039 |
| Popis: | An analytical procedure is described, which allows the simultaneous determination of 22 pesticides in drinking, ground and surface water. In case of higher-loaded waters the sample has to be submitted to a clean-up step after CH2Cl2 extraction. The following HPLC is carried out on a Hypersil-column, 3 μm ODS by gradient elution using CH3CN-H2O (phosphate buffer, pH 7) as eluent. The detection of the single components is achieved by a diode-array detector according to their absorption maximal at 220, 230, 245 nm, the verification being supported by comparison of the absorption spectra. As the factor of enrichment is about 1000 a detection limit of 50 μg/l for any pesticide can be obtained. The recovery is higher than 90%. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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