HPLC determination of the phenolic metabolites of phenoxy alkanoic acid herbicides

Autor: T. J. El-Ahmed, M. A. Alawi, M. K. Fayyad
Rok vydání: 1989
Předmět:
Zdroj: Chromatographia. 28:465-468
ISSN: 1612-1112
0009-5893
DOI: 10.1007/bf02261061
Popis: High performance liquid chromatography (HPLC) has been applied to the separation and determination of o-chlorophenol, 2,4-dichlorophenol, 3,4-dichlorophenol, 2,5-dichlorophenol, and 2,4,5-trichlorophenol in water samples after prior derivatization with 4-aminoantipyrine. These substances are the phenolic decomposition products of the phenoxy-alkanoic acid herbicides: 2,4-dichlorophenoxyacetic acid (2,4-D), 2,4-dichlorophenoxybutyric acid (2,4-DB), and 2,4,5-trichlorophenoxyacetic acid (2,4,5-T). An RP-C18 column was used, with gradient elution starting at 50/50 and changing to 70/30 methanol/water (v/v) after 24 min. Detection was carried out at 470 nm where the 4-aminoantipyrilquinonimine derivatives show maximum absorbance. The method was linear over a concentration range of 5–10,000 ppb phenol in the original water sample. By using an enrichment step a detection limit of 2 ppb phenol could be approached. The method was used to study the degree of degradation of the phenoxy acids to phenols under different conditions of temperature, pH, and UV irradiation.
Databáze: OpenAIRE
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