Effects of low-intensity pulsed ultrasound on proliferation and chondroitin sulfate synthesis of cultured chondrocytes embedded in Atelocollagen® gel

Autor: H. Kataoka, Yuji Uchio, Masakazu Kuriwaka, T. Nishikori, Mitsuo Ochi, Kenzo Kawasaki, Kenichi Katsube, S. Maniwa
Rok vydání: 2001
Předmět:
Zdroj: Journal of Biomedical Materials Research. 59:201-206
ISSN: 0021-9304
DOI: 10.1002/jbm.1226
Popis: The effects of low-intensity pulsed ultrasound (US) on the proliferation and chondroitin sulfate synthesis of cultured chondrocytes embedded in Atelocollagen® gel in vitro were examined. Articular cartilage was harvested from the hip, knee, and shoulder joints of 10-week-old Japanese white rabbits. Chondrocytes isolated by collagenase digestion were embedded in type I collagen gel, Atelocollagen gel, and were cultured in Dulbecco's modified eagle's medium for 3 weeks. The US apparatus, SAFHS® , was used to deliver an ultrasound signal with spatial and temporal average intensities of 30 mW/cm2 (US group). The frequency was 1.5 MHz with a 200-microsecond tone burst repeated at 1.0 kHz. US treatments were administered for 20 min per day under culture dishes, with the medium replaced twice a week. Another group of cells was exposed to sham ultrasound as a control. Cell number, histological findings, synthesis of isomers of chondroitin sulfate, and stiffness of the chondrocyte–collagen gel composites were analyzed. US exposure promoted synthesis of chondroitin sulfate, especially chondroitin 6-sulfate, although it did not significantly enhance cell number and stiffness. In this three-dimensional culture model, these results suggest that US exposure may be clinically useful in improving the quality of chondrocyte–Atelocollagen implants for transplantation into articular cartilage defects. © 2001 Wiley Periodicals, Inc. J Biomed Mater Res 59: 201–206, 2002
Databáze: OpenAIRE
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