In vitro propagation of the endangered tree fern Cibotium barometz through formation of green globular bodies

Autor: Zhou Xuhong, Han Li, Hua Cao, Shenchong Li, Guangfei Zhang, Yu Rongpei, Yalian Jiang, Wang Jihua, Gui Min, Mo Xijun
Rok vydání: 2016
Předmět:
Zdroj: Plant Cell, Tissue and Organ Culture (PCTOC). 128:369-379
ISSN: 1573-5044
0167-6857
DOI: 10.1007/s11240-016-1116-0
Popis: Cibotium barometz is an endangered tree fern, used both as ornamental plant and traditional Chinese medicinal plant. In this study, an effective in vitro propagation protocol was obtained through formation of green globular bodies (GGBs) from in vitro juvenile sporophytes. The effect of plant growth regulators (PGRs) on GGB induction and multiplication, as well as mineral salt concentration and active charcoal (AC) on plantlet regeneration from GGBs was evaluated. Thidiazuron (TDZ; 1-phenyl-3-(1,2,3-thiadiazol-5-yl) urea) had a significant effect on GGB induction and multiplication (P 0.05). GGB induction rate was above 80 % on 1/2 Murashige and Skoog (MS) media supplemented with TDZ (1.0 mg L− 1) and NAA (0.1, 0.3 or 0.5 mg L− 1). The same media were also optimal for GGB multiplication. GGBs cultured on 1/4 MS media supplemented with 0.1 or 0.2 % (w/v) AC showed a high rate of GGB development into plantlets above 90 %. 1/2 MS media supplemented with 0.1 or 0.2 % AC were the most effective for plantlet growth. Regenerated plantlets were successfully acclimatized (80 %) in greenhouse conditions. Morphological and histological analysis revealed that C. barometz GGBs was a yellow-green globular structure composed of the single GGB with meristems and hair-like structures, and new single GGBs were initiated from the epidermal cells of meristem zone.
Databáze: OpenAIRE
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