Choriocarcinoma-trophoblast hybrid cells: Reconstructing the pathway from normal to malignant trophoblast — Concept and perspectives
Autor: | Hans-Georg Frank, Hitoshi Funayama, Ulrike Schmitz, Gabi Gaus |
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Rok vydání: | 1999 |
Předmět: |
Genetics
education.field_of_study Choriocarcinoma Population Obstetrics and Gynecology Trophoblast Biology medicine.disease medicine.disease_cause Phenotype female genital diseases and pregnancy complications In vitro Malignant transformation medicine.anatomical_structure Reproductive Medicine embryonic structures medicine Cancer research Carcinogenesis education Gene reproductive and urinary physiology Developmental Biology |
Zdroj: | Placenta. 20:11-24 |
ISSN: | 0143-4004 |
Popis: | Summary Extravillous trophoblast cells of normal and molar pregnancies spontaneously can transform into choriocarcinoma cells. This malignant transformation differs from that in other tissues in two respects: (a) the normal tissue of origin, extravillous trophoblast, is already invasive, and (b) both, choriocarcinoma and extravillous trophoblast, are foreign (allohaploid) to the host. Carcinogenetic transformation of trophoblast into choriocarcinoma is a multistep process in the course of which temporary intermediate geno- and phenotypes are generated; these are mixtures between trophoblast and choriocarcinoma and illustrate the process of carcinogenesis. The intermediates are either selected by the host for survival or undergo further mutations. Consequently, it remains unknown which properties are lost or acquired during the process and which of them are crucial either for control of invasion of normal extravillous trophoblast or for the malignant development of choriocarcinoma cells. In order to identify mutative trophoblast-relevant oncogenes and tumor suppressor genes, we have generated somatic cell hybrids using the choriocarcinoma cell line AC1-1 and extravillous trophoblast cells. The resulting hybrid cells were cloned, characterized cytogenetically and classified according to tumorigenicity. These hybrids represent a population of intermediates between non-tumorigenic extravillous trophoblast cells and highly tumorigenic choriocarcinoma cells, similar to those temporarily formed during carcinogenesis. The hybrids are thought to represent a suitable in vitro tool for the identification of genes and gene products involved in both control of normal invasion and malignant transformation of trophoblast cells. Analysis of the hybrid clones involves both, methods assessing phenotypic (functional) and genotypic properties. |
Databáze: | OpenAIRE |
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