The design of agarose beds for high-performance hydrophobic-interaction chromatography and ion-exchange chromatography which show increasing resolution with increasing flow rate
| Autor: | Yao Kunquan, Stellan Hjertén, Jia-Li Liao |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Chromatography
Polymers and Plastics Resolution (mass spectrometry) Chemistry Hydrophilic interaction chromatography Organic Chemistry Ion chromatography technology industry and agriculture Condensed Matter Physics High-performance liquid chromatography Volumetric flow rate Hydrophobic effect chemistry.chemical_compound Materials Chemistry Agarose Porous medium |
| Zdroj: | Makromolekulare Chemie. Macromolecular Symposia. 17:349-357 |
| ISSN: | 0258-0322 |
| DOI: | 10.1002/masy.19880170126 |
| Popis: | Agarose beads (agarose concentration: 15%; diameter: 15–70 μm) were shrunk, crosslinked and derivatized in organic solvents. As crosslinker and coupling agent γ-glycidoxypropyl tri-methoxysilane was used. Columns packed with nonporous beads of pentyl agarose and octyl agarose, prepared by this technique, were used for hydrophobic-interaction chromatography. Characteristic of these columns was that the resolution increased with an increase in flow rate (except for very low flow rates). This very attractive behavior, which violates the generally accepted theory of chromatography, was also exhibited by an ion-exchanger based on non-porous agarose beads. |
| Databáze: | OpenAIRE |
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