Double-antibody enzyme immunoassay for nortriptyline

Autor:	E. Gnemmi, Vincent Marks, M.J. O'Sullivan, M N Al-Bassam, J W Bridges
Rok vydání:	1978
Předmět:	chemistry.chemical_classification Chromatography biology medicine.diagnostic_test Chemistry Biochemistry (medical) Clinical Biochemistry Substrate (chemistry) Radioimmunoassay Enzyme assay Enzyme Dimethyl Adipimidate Reagent Immunoassay biology.protein medicine Nortriptyline medicine.drug
Zdroj:	Clinical Chemistry. 24:1590-1594
ISSN:	1530-8561 0009-9147
DOI:	10.1093/clinchem/24.9.1590
Popis:	beta-D-Galactosidase (EC 3.2.1.23) from Escherichia coli was conjugated to desmethylnortriptyline by means of a bifunctional cross-linking reagent, dimethyl adipimidate, and used in a double-antibody immunoassay for nortriptyline. Eighty percent of the enzyme activity was retained after conjugation; 75% of the enzyme was conjugated to desmethylnortriptyline. In the final immunoassay the enzyme activity of the bound fraction was determined with o-nitrophenyl-beta-D-galactopyranoside as substrate. The sensitivity, precision, and simplicity of the enzyme immunoassay compared favorable with that of a published radioimmunoassay method. Results for nortriptyline in plasma samples correlated well with those determined by either radioimmunoassay or gas-chromatography.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::730546c53d4245895607fb19dd381821 https://doi.org/10.1093/clinchem/24.9.1590 Zobrazit plný text záznamu