The Intracellular Sorting of Myosin Essential Light Chain Isoproteins in Chicken Fibroblasts

Autor:	Muhammad Mujahid Khan, Masatoshi Komiyama, Yutaka Shimada
Rok vydání:	1997
Předmět:	Gene isoform Cardiac muscle General Physics and Astronomy Skeletal muscle General Medicine Biology Molecular biology medicine.anatomical_structure Cytoplasm Myosin medicine MYH7 General Agricultural and Biological Sciences Myofibril Fibroblast
Zdroj:	Proceedings of the Japan Academy, Series B. 73:219-222
ISSN:	1349-2896 0386-2208
DOI:	10.2183/pjab.73.219
Popis:	In order to investigate the sorting specificity of myosin essential light chain (ELC) isoproteins for myosin heavy chain (MHC) of the nonmuscle type of stress fibers, epitope-tagged ELC cDNAs of the nonmuscle and fast skeletal muscle types were force-expressed in cultured cardiac fibroblasts from chicken embryos. Although the expressed ELC of the former type was found in the cytoplasm and nucleus as well as on stress fibers, that of the latter was found only along stress fibers. Thus, compared to ELC of the nonmuscle type, that of the fast skeletal muscle type has a higher affinity for the nonmuscle MHC present endogenously in fibroblast stress fibers. Together with the observation that this isoform has the highest affinity for MHC of the cardiac muscle type in myofibrils of caridomyocytes (Komiyama et al., 1996, J. Cell Sci. 109, 2089-2099), it can be concluded that ELC of the fast skeletal muscle type is an evolved isoform possessing the ability to associate tightly with a variety of MHC isoforms.
Databáze:	OpenAIRE
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