Association of interleukin-18 gene promoter polymorphisms with coronary artery disease in northern Iranian population
Autor: | Majid Shahbazi, Touraj Farazmandfar, Aref Salehi, Marzieh Attar, Afsaneh Bazgir, Reza Salehi Manzari |
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Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
medicine.medical_specialty business.industry medicine.disease Gastroenterology Angina Pathogenesis Coronary artery disease 03 medical and health sciences 030104 developmental biology Internal medicine Genotype Genetics medicine Interleukin 18 Myocardial infarction Allele business Genotyping Genetics (clinical) |
Zdroj: | Meta Gene. 16:1-4 |
ISSN: | 2214-5400 |
Popis: | Background Coronary artery disease (CAD) is the leading cause of disability and mortality in the world. CAD includes a group of cardiovascular diseases such as angina, heart failure and myocardial infarction. Interleukin-18 (IL-18) is a pleiotropic proinflammatory cytokine and an inflammation inducer, which is encoded by the IL-18 gene located on chromosome 11. Different studies have shown the involvement of IL-18 in the pathogenesis of diseases such as atherosclerosis. Objective This study investigated the association of the IL-18 gene promoter polymorphisms (− 137 and − 607) with CAD in northern Iranian population. Methods Genomic DNA was extracted from peripheral blood of 314 patients with CAD and 364 healthy controls by a standard protocol. Sequence-Specific Primer-PCR was used for genotyping. Total RNA was extracted from peripheral blood leukocytes from 80 patients with CAD and 80 healthy individuals using Trizol reagent. Real-time PCR was used to measure the level of IL-18 mRNA in the patients and controls. Results Analysis of genotypes revealed that the frequencies of genotype G/C and allele C in IL-18(− 137) polymorphism were significantly lower in patients group than control group (p = 0.019 and p = 0.028 respectively). No significant difference was found in the IL-18(− 607) between the patients and controls. Moreover, the level of IL-18 expression was significantly higher in CAD patients than in controls (p Conclusion The results of the present study suggested a protective effect for genotype G/C of IL-18(− 137) in CAD. Moreover, due to an increased expression level of IL-18 in CAD patients, it could be considered as a prognostic and protective factor in this disease. |
Databáze: | OpenAIRE |
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