Association of interleukin-18 gene promoter polymorphisms with coronary artery disease in northern Iranian population

Autor: Majid Shahbazi, Touraj Farazmandfar, Aref Salehi, Marzieh Attar, Afsaneh Bazgir, Reza Salehi Manzari
Rok vydání: 2018
Předmět:
Zdroj: Meta Gene. 16:1-4
ISSN: 2214-5400
Popis: Background Coronary artery disease (CAD) is the leading cause of disability and mortality in the world. CAD includes a group of cardiovascular diseases such as angina, heart failure and myocardial infarction. Interleukin-18 (IL-18) is a pleiotropic proinflammatory cytokine and an inflammation inducer, which is encoded by the IL-18 gene located on chromosome 11. Different studies have shown the involvement of IL-18 in the pathogenesis of diseases such as atherosclerosis. Objective This study investigated the association of the IL-18 gene promoter polymorphisms (− 137 and − 607) with CAD in northern Iranian population. Methods Genomic DNA was extracted from peripheral blood of 314 patients with CAD and 364 healthy controls by a standard protocol. Sequence-Specific Primer-PCR was used for genotyping. Total RNA was extracted from peripheral blood leukocytes from 80 patients with CAD and 80 healthy individuals using Trizol reagent. Real-time PCR was used to measure the level of IL-18 mRNA in the patients and controls. Results Analysis of genotypes revealed that the frequencies of genotype G/C and allele C in IL-18(− 137) polymorphism were significantly lower in patients group than control group (p = 0.019 and p = 0.028 respectively). No significant difference was found in the IL-18(− 607) between the patients and controls. Moreover, the level of IL-18 expression was significantly higher in CAD patients than in controls (p Conclusion The results of the present study suggested a protective effect for genotype G/C of IL-18(− 137) in CAD. Moreover, due to an increased expression level of IL-18 in CAD patients, it could be considered as a prognostic and protective factor in this disease.
Databáze: OpenAIRE