Purification and characterization of DNase VII, a 3‘ leads to 5‘-directed exonuclease from human placenta
Autor: | G F Hollis, L Grossman |
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Rok vydání: | 1981 |
Předmět: | |
Zdroj: | Journal of Biological Chemistry. 256:8074-8079 |
ISSN: | 0021-9258 |
Popis: | An exonuclease, DNase VII, has been purified 6000-fold from human placenta. The enzyme has an apparent molecular weight of 43,000, requires Mg2+ for activity, and has a pH optimum of 7.8. The enzyme hydrolyzes single-stranded and nicked duplex DNA at the same rate proceeding in a 3' leads to 5' direction liberating 5'-mononucleotides. It does not measurably hydrolyze polyribonucleotides. |
Databáze: | OpenAIRE |
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