Purification and characterization of DNase VII, a 3‘ leads to 5‘-directed exonuclease from human placenta

Autor:	G F Hollis, L Grossman
Rok vydání:	1981
Předmět:	chemistry.chemical_classification Exonuclease Dna duplex biology Ph optimum Polyribonucleotides Human placenta Cell Biology Biochemistry Molecular biology Enzyme medicine.anatomical_structure chemistry Placenta biology.protein medicine Deoxyribonucleases Molecular Biology
Zdroj:	Journal of Biological Chemistry. 256:8074-8079
ISSN:	0021-9258
Popis:	An exonuclease, DNase VII, has been purified 6000-fold from human placenta. The enzyme has an apparent molecular weight of 43,000, requires Mg2+ for activity, and has a pH optimum of 7.8. The enzyme hydrolyzes single-stranded and nicked duplex DNA at the same rate proceeding in a 3' leads to 5' direction liberating 5'-mononucleotides. It does not measurably hydrolyze polyribonucleotides.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::72c8a9ba2023d11b293a05991fc44c47 https://doi.org/10.1016/s0021-9258(18)43390-x Zobrazit plný text záznamu