Determination of Candida albicans fungus proteins concentration by Elisa method at Intramuscular Introduction in Candidiasis Therapy
| Autor: | Mykola Rybalkin, Serhiy Stepanenko, Natalia Khokhlenkova, Tatiana Diadiun, Yuliia Azarenko |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
biology business.industry 030106 microbiology Fungus biology.organism_classification Microbiology 03 medical and health sciences 030104 developmental biology Antigen Immunity Medicine Pharmacology (medical) Elisa method business Candida albicans Pharmacology Toxicology and Pharmaceutics (miscellaneous) |
| Zdroj: | Research Journal of Pharmacy and Technology. :3249-3252 |
| ISSN: | 0974-360X 0974-3618 |
| DOI: | 10.52711/0974-360x.2021.00565 |
| Popis: | The objective of this work was to determine the C. albicans fungus protein concentration at intramuscular introduction in therapy of candidiasis. Therapeutic effect of C. albicans fungus proteins in concentrations 1, 2, 3, 4 and 5mg/mL has been examined in white mice. Animals were infected intraperitoneally with suspension of C. albicans fungus strain CCM 335-867 in the amount of 20 million cells per 1mL volume. After 5 days and repeatedly after 14 days mice were injected intramuscularly in the upper back hind paw with proteins of Candida fungus cells of volume 0.2mL. 14 days after each injection, the determination of the protective functions of the animal body has been carried out by the titer of specific C. albicans antibodies during immunoassay. According to the data obtained during studies on the treatment of candidiasis, it has been found that in the intramuscular route of administration after the first injection with C. albicans protein concentration of 3 mg/mL antibody titers increased 2 times, and after the second - 8 times. Proteins of C. albicans fungus at a concentration of 3 mg/mL activate the body's defence mechanisms. |
| Databáze: | OpenAIRE |
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