Glyphosate resistance in Ambrosia trifida : Part 1. Novel rapid cell death response to glyphosate
| Autor: | Peter H. Sikkema, Dafu Wang, R. Douglas Sammons, Marcelo L. Moretti, Bryan G. Young, Renae R. Robertson, Mackenzie A Lespérance, Todd A. Gaines, Michael D. McLean, Burkhard Schulz, Mark B Lawton, J. Christopher Hall, Stephen C. Weller, Amanda C Green, Christopher R Van Horn, François J. Tardif, Taylor Jeffery, Philip Westra, William G. Johnson, Kabelo Segobye |
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| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
Genetics Programmed cell death biology Resistance (ecology) 04 agricultural and veterinary sciences General Medicine biology.organism_classification 01 natural sciences Phenotype chemistry.chemical_compound Agronomy Ambrosia trifida chemistry Insect Science Glyphosate 040103 agronomy & agriculture Rr- phenotype 0401 agriculture forestry and fisheries Agronomy and Crop Science After treatment Rapid response 010606 plant biology & botany |
| Zdroj: | Pest Management Science. 74:1071-1078 |
| ISSN: | 1526-498X |
| Popis: | BACKGROUND Glyphosate-resistant (GR) Ambrosia trifida is now present in the midwestern United States and in southwestern Ontario, Canada. Two distinct GR phenotypes are known, including a rapid response (GR RR) phenotype, which exhibits cell death within hours after treatment, and a non-rapid response (GR NRR) phenotype. The mechanisms of resistance in both GR RR and GR NRR remain unknown. Here, we present a description of the RR phenotype and an investigation of target-site mechanisms on multiple A. trifida accessions. RESULTS Glyphosate resistance was confirmed in several accessions, and whole-plant levels of resistance ranged from 2.3- to 7.5-fold compared with glyphosate-susceptible (GS) accessions. The two GR phenotypes displayed similar levels of resistance, despite having dramatically different phenotypic responses to glyphosate. Glyphosate resistance was not associated with mutations in EPSPS sequence, increased EPSPS copy number, EPSPS quantity, or EPSPS activity. CONCLUSION These encompassing results suggest that resistance to glyphosate in these GR RR A. trifida accessions is not conferred by a target-site resistance mechanism. © 2017 Society of Chemical Industry |
| Databáze: | OpenAIRE |
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