Radial multi-stationary phase thin-layer chromatography for the field-ready fingerprinting of herbal materials
| Autor: | Erwin P. Enriquez, Isagani D. Padolina, Sarah May Sibug-Torres |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Materials science
Chromatography biology Elution Silica gel General Chemical Engineering 010401 analytical chemistry General Engineering Single sample 02 engineering and technology 021001 nanoscience & nanotechnology biology.organism_classification 01 natural sciences Thin-layer chromatography 0104 chemical sciences Analytical Chemistry chemistry.chemical_compound chemistry Stationary phase Plant species Blumea balsamifera 0210 nano-technology Control methods |
| Zdroj: | Analytical Methods. 11:5511-5520 |
| ISSN: | 1759-9679 1759-9660 |
| DOI: | 10.1039/c9ay01714c |
| Popis: | The growing international demand for herbal-based products has prompted the need for more stringent quality control methods to detect substandard and adulterated herbal plant materials. However, most prescreening methods, such as thin-layer chromatography (TLC), remain inaccessible to herbal producers in resource-limited settings. Here, we report a proof-of-concept demonstration of a multi-stationary phase thin-layer chromatography (MSP-TLC) method for the preliminary on-site identification and evaluation of herbal materials. Our method is based on a unique TLC plate design that features multiple phenyl- and octyl-modified silica gel stationary phases configured as radial sectors. The modified stationary phase patterns were fabricated by screen-printing organosilane solutions onto commercial silica gel TLC plates. Radial elution with an ethanol–water mobile phase from the center of the MSP-TLC plate generates multiple chromatographic profiles simultaneously for a single sample extract. To facilitate the interpretation of the multiple TLC profiles, the MSP-TLC system was coupled with image analysis and chemometric pattern recognition to classify a sample as “within-specifications” or “off-specifications” for a given herbal plant species. Application of the system to Blumea balsamifera and Vitex negundo demonstrated sensitivity and specificity rates that range from 73.1 to 95.1% compared to the respective standard Pharmacopeia TLC methods. The presented method holds considerable promise as a cost-effective, user-friendly on-site prescreening tool for herbal materials in resource-limited settings. |
| Databáze: | OpenAIRE |
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