Studies on the proteins involved in the interaction of high‐density lipoprotein with isolated human small intestine epithelial cells

Autor:	Tenkanen H, Dmitri Sviridov, Vadim S. Repin, Pavlov MYu, Christian Ehnholm, Safonova Ig
Rok vydání:	1992
Předmět:	biology Apolipoprotein B Enterocyte Binding protein Apolipoprotein A-II Biophysics Cell Biology Biochemistry medicine.anatomical_structure Structural Biology Polyclonal antibodies Genetics biology.protein medicine lipids (amino acids peptides and proteins) Binding site Antibody Molecular Biology Lipoprotein
Zdroj:	FEBS Letters. 303:202-204
ISSN:	0014-5793
DOI:	10.1016/0014-5793(92)80519-m
Popis:	Treatment of125I-labelled high-density lipoprotein ([125I]HDL3) with monospecific polyclonal antibodies against apolipoproteins A-I and A-II resulted in a dose-dependent inhibition of the [125I]HDL3 binding to isolated human small intestine epithelial cells by 25%, and 50%, respectively. Both antibodies also inhibited intracellular degradation of [125I]HDL3 by 80%. Treatment of enterocytes with polyclonal antibody against apolipoprotein A-I binding protein, a putative HDL receptor, inhibited both binding and degradation of [125I]HDL3 by these cells by 50%. Antibodies to apolipoprotein A-I, A-II and apo A-I-binding protein also inhibited [125I]HDL3 binding to cholesterol-loaded cells.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::72ad904e3eb6b9cca6fbaf72c9d45cfe https://doi.org/10.1016/0014-5793(92)80519-m Zobrazit plný text záznamu Plný text