| Autor: | Dennis Pitt, C. Brownlee, Michael Moore, R. D. Holland |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Neutral red
Fucus serratus Acid phosphatase Cell Biology Biology biology.organism_classification Esterase Lysosomal acid phosphatase chemistry.chemical_compound Human fertilization medicine.anatomical_structure Vital stain chemistry Biochemistry Lysosome embryonic structures biology.protein medicine Anatomy |
| Zdroj: | The Histochemical Journal. 29:239-248 |
| ISSN: | 0018-2214 |
| DOI: | 10.1023/a:1026406010587 |
| Popis: | Fucus serratus eggs were examined for evidence of the existence of a lysosome-like body using enzyme histochemical and vital staining techniques. Simultaneous coupling azo-dye techniques for lysosomal acid phosphatase proved inappropriate owing to endogenous phenolic binding artefacts. The large number of alginate polysaccharide and polyphenolic egg vesicles interfered with vital staining techniques for lysosomes. Lysosomal esterase activity was detected in the abundant egg lipid bodies. The role of the egg lipid body as an equivalent lysosome-like body of higher plants, the spherosome, is discussed in relation to egg fertilization and early zygote development. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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