| Popis: |
The preparatory techniques of freeze substitution for electron microscopy are given in detail. Included is a simple, reliable method for the selection of individual freeze-substituted cells from flat embedments via light microscopy for subsequent electron microscope analysis. These mthods are intended for studying cells in monolayer cultures, tissues, suspensions of cells, or cell fractions and are widely applicable for cell biologists in any field. An historical background and review of the relevant literature is included so that the reader will be fully prepared to use of modify the techniques and to troubleshoot results as required. All materials that are needed are thoroughly discussed so that any laboratory can adopt freeze substitution as a routine procedure. |
