Testing the purity of cell cultures using clinical diagnostic PCR kits
| Autor: | A. Yu. Malinin, S.N. Borchsenius, I. E. Vishnyakov, A. V. Morozova |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Quantitative Evaluations Cell Biology Mycoplasma hominis Biology Human cell Contamination Diagnostic system biology.organism_classification Virology DNA sequencing 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Cell culture 030220 oncology & carcinogenesis Viral load |
| Zdroj: | Cell and Tissue Biology. 11:250-259 |
| ISSN: | 1990-5203 1990-519X |
| DOI: | 10.1134/s1990519x17030075 |
| Popis: | Cell cultures of higher organisms, especially human cells, are increasingly used in medical, pharmaceutical, and scientific research. The main difficulty for cell cultures is hidden nonlethal contamination by mycoplasmas, viruses, and cross contamination with other cells. We suggest using PCR kits designed and officially employed in clinical diagnostics as an accessible and reliable method for monitoring the purity of cell cultures. We tested 50 human cell lines using commercial diagnostic systems for detection of papillomaviruses, herpes viruses, adenoviruses, Mycoplasma hominis, and total bacterial mass. No contamination was revealed in the cell lines tested. For the cell lines that contained integrated parts of viral genomes, the presence of the corresponding DNA sequences was confirmed. These diagnostic systems can be effectively used to test the purity of cell lines and for qualitative detection of possible contamination, as well as for quantitative evaluations using calculation of viral load, just as is practiced in clinical diagnostics. |
| Databáze: | OpenAIRE |
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