| Autor: |
Yu-An Sui, Satoshi Maruyama, Natsumi Okada, Masakazu Ito, Masayoshi Muramatsu, Shusei Obata, Jun Ogawa, Shigenobu Kishino |
| Rok vydání: |
2023 |
| Předmět: |
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| Zdroj: |
Bioscience, Biotechnology, and Biochemistry. |
| ISSN: |
1347-6947 |
| DOI: |
10.1093/bbb/zbad056 |
| Popis: |
PsADH, an alcohol dehydrogenase originating in Pantoea sp. was characterized and found to convert a broad variety of fatty alcohols into their corresponding aldehydes, the substrates of alkane biosynthesis. By coupling PsADH with NpAD, a cyanobacterial aldehyde-deformylating oxygenase, and by optimizing the conditions of the enzyme-catalyzed reactions, we achieved a 52% conversion of 1-tetradecanol to tridecane. We further applied this system to generate alkanes ranging from C5–17. These alkanes can be used as biofuels, suggesting that introducing a suitable alcohol dehydrogenase is an effective strategy to utilize fatty alcohols for alkane production. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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