Carbonic anhydrase: Its content in spinach leaves and its taxonomic diversity studied with anti-spinach leaf carbonic anhydrase antibody
| Autor: | Shi-Yuan Yang, Mikio Tsuzuki, Shigetoh Miyachi, Keiichiro Okabe |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
chemistry.chemical_classification
biology food and beverages Soil Science Chlamydomonas reinhardtii biology.organism_classification Ouchterlony double immunodiffusion Enzyme Biochemistry chemistry Carbonic anhydrase Botany biology.protein Crassulacean acid metabolism Spinach Agronomy and Crop Science Solanaceae Nicotiana |
| Zdroj: | Plant Science Letters. 33:145-153 |
| ISSN: | 0304-4211 |
| DOI: | 10.1016/0304-4211(84)90004-x |
| Popis: | Carbonic anhydrase (CA; EC 4.2.1.1) was purified to electrophoretic homogeneity from spinach leaves. With this enzyme, anti-spinach CA antibody was prepared using rabbits. This antibody precipitated almost all CA activity from a crude extract of spinach leaves. Various immunochemical determinations indicated that the CA protein content in spinach leaves was 1–2% of the total soluble leaf protein. Ouchterlony double immunodiffusion analysis indicated cross-reactivity with leaf extracts from several terrestrial C3 plants, C4 dicotyledons and Crassulacean acid metabolism (CAM) dicotyledons, while the extracts from several C4 monocytyledons and the green alga, Chlamydomonas reinhardtii, showed no cross-reactivity with the anti-spinach leaf CA antibody. |
| Databáze: | OpenAIRE |
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